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Apr 01, 1986#0183;#32;Cell disruption in bead mills is regarded as one of the most efficient techniques for physical cell dis ruption .23 Various designs of bead mills have been used for microbial cell disruption,ls''33''~ss These mills consist of either a vertical or a horizontal cylindrical chamber with a motordriven central shaft supporting a collection of off
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Mar 01, 1983#0183;#32;The usefulness of the Netzsch LME 20mill for the disruption of bacteria species was demonstrated with Escherichia coli, Brevibacterium ammoniagenes, Bacillus sphaericus and Lactobacillus confusus. As expected, the mill capacity for bacterial disruption was significantly smaller than for the yeast.
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Cell disruption can be brought about by a highspeed bead mill, consisting of a series of rotating discs and a charge of glass ballotini (Figure 2).The cell suspension is agitated at a very high speed. The mill chamber is almost full of grinding beads during operation, with the optimum concentration of ballotini usually being 7090% of the volume of the chamber.
#0183;#32;Cell disruption in bead mills is regarded as one of the most efficient techniques for physical cell dis ruption .23 Various designs of bead mills have been used for microbial cell disruption,ls''33''~ss These mills consist of either a vertical or a horizontal cylindrical chamber with a motordriven central shaft supporting a collection of off centred discs or other agitating element
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This increases the disruption of microbial cells albeit at the expense of higher power input and heat generation. 27, 43 Smaller microbial cells, such as bacterial cells, generally require higher
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